How do sperm penetrate chicken eggs

The chicken egg-envelope is made of two major glycoprotein components, which are designated as gp97 and gp42 after their molecular masses. To elucidate how these two components are involved in macromolecular organization of the chicken egg-envelope, the isolated egg-envelope was characterized by immunochemical and biochemical methods. The gp97 was suggested to be a homologue of mouse ZPB based on the similarities of N-terminal and internal sequences. Immunoblotting using anti-gp97 monoclonal antibodies and two-dimensional gel electrophoresis with or without mercaptoethanol treatment revealed that gp97 formed a homodimer through disulfide bonds, whereas gp42 did not. Under indirect immunofluorescence microscopy, the anti-gp97 antibody visualized indistinct, small spots on the egg-envelope, whereas the anti-gp42 antibody showed a meshwork of blurry, fibrous structures.
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Hen reproduction

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How Do Chickens Fertilize Eggs? | Sciencing

Effective date : Year of fee payment : 4. Year of fee payment : 8. The present invention relates to the field of avian egg fertilization.
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Chicken Reproduction – Sperm & Eggs

Chickens — like other birds — lay fertilized eggs via sexual reproduction. Depending on the breed of chicken, a hen will begin laying eggs between five and seven months of age. The frequency of egg-laying varies between breeds, over different seasons, with moulting and with age, but most breeds that are used for egg production will lay an egg every one to two days.
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Keeping Chickens. I understand, and perhaps I misunderstand, that the sperm of the rooster is stored in the hens body until a egg passes by and then it is fertilized? So does a rooster deposit sperm that effects several eggs, or must he make a fresh deposit for each egg? A mature ovary, which looks like a cluster of grapes, may contain up to 4, small ova.
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